β-HYDROXYGLUTAMIC ACID DECARBOXYLASE

Enzymic reactions in mammalian metabolism of y-hydroxyglutamic acid

Glyoxylic acid has recently been shown to be formed from 7-hydroxyglutamate by an enzyme system present in rat-liver extracts z. Paper-chromatographic identification of alanine as one other product of this process suggested the possibilities of either a direct cleavage of the substrate or a series of reactions with formation of alanine as a terminal product. The present experiments show that th...

Aromatic LAmho Acid Decarboxylase*

After the finding of 5-hydroxytryptamine (serotonin) in tissues (2), this laboratory reported that the amine is derived from the dietary amino acid, tryptophan (3), 5-hydroxytryptophan being the immediate precursor. Decarboxylation of 5-hydroxytryptophan was shown to be catalyzed by an enzyme found in many tissues and was originally considered to be distinct from 3,4-dihydroxyphenylalanine deca...

Observations on cysteic acid decarboxylase.

Glutamic acid decarboxylase in Rhodotorula glutinis.

1. The metabolism of 131I-labelled thyroglobulin from four mammalian species has been studied. 2. The rat, dog and monkey rapidly metabolize the homologous protein, with the formation of iodide, iodotyrosines and thyroxine. Rabbit thyroglobulin disappears slowly from the plasma of the rabbit after intravenous injection, and no metabolites have so far been detected in the blood. 3. The developme...

Monoclonal antibodies to glutamic acid decarboxylase.

Five monoclonal antibodies that recognize chicken brain glutamic acid decarboxylase (GAD) have been selected and designated GAD-1 to -5. GAD-1 to -5 were selected on the basis of their ability to immunoprecipitate active GAD from crude brain extracts. GAD-1 recognizes an epitope that is conserved in many vertebrates; the epitope recognized by GAD-5 is restricted to the chicken. Radioimmunoassay...